Search
Identification of new causative genes in spinocerebellar degenerations by combination of whole genome scan, next-generation sequencing and biological validation in vitro and in vivo – SPATAX-QUEST
the most promising variants in vitro (Partner 1, cell models) and in vivo by modeling and rescue experiments [...] explore their dynamics of interaction in vitro (Partner 1) and in vivo using C. elegans (Partner 4). A systematic
Determining factors of thea difference in toxicity in intestine and liver for two similar sized nanoparticles used in food and packaging: In vitro and in vivo investigation on uptake and mechanisms involved – SolNanoTOX
MNMs following oral exposure in vivo. Nevertheless, results from in vivo experiments are the main data [...] mechanisms, the project combines integrative in vitro and in vivo approaches to compare the fate, cytogenotoxic [...] investigated in vivo after short-term oral treatment of rodents and compared to in vitro data obtained
Development of a lanthanide-based luminescent nanoparticule for the dynamic quantification of autophagy in vitro and in vivo. – Lumiphage
e informations on the autophagic flux in vitro and in vivo non-invasively. The autophagic flux is described [...] to image and quantify non-invasively autophagy in vivo will have an important impact on therapeutic efficiency [...] developped to study the functions of autophagy in vitro, molecules that can be exploited to evaluate dynamically
DEAD-box RNA helicases: in vivo roles, protein partners, RNA substrates and mechanistic details – HeliDEAD
RNA helicases are ubiquitous proteins found in all three kingdoms of life. They are NTP-dependent RNA binding proteins and RNA-dependent NTPases that are associated with all cellular processes involving RNA. Yeast has 45 different RNA helicases that are generally essential and rarely interchangeable, and they are functionally conserved throughout the eukaryotes. Our laboratory is interested in understanding how RNA helicases work, what are their cellular roles and how they are regulated.
Structural and functional studies of mitoNEET. from molecules to in vivo studies – MIdiaZONE
The discovery of the Fe-S protein mitoNEET at the crossroad of the mitochondrial outer membrane and the cytosol has opened up new avenues for understanding maturation of extra-mitochondrial Fe-S proteins in mammalian cells. Investigations performed in the framework of this project will tackle unanswered questions regarding Fe-S transfer to cytosolic recipients. Characterizing mitoNEET-pioglitazone interactions should open up perspectives for improving efficacy of thiazole and thiophene drugs.
Spatially-restricted transgene expression in vivo by two-photon release of small molecule inducers – LocaGenEx
has been successfully demonstrated in vitro. However, in vivo applications are experimentally much more [...] targeted transgene expression in single cells in vivo by irradiation with two-photon light so that the [...] properties according to the experimental needs in vivo. The goal is to photoactivate Kir2.1, a gene that
Chaperone-mediated control of toxin-antitoxin systems in Mycobacterium tuberculosis. – mycoTAC
Chaperone-mediated control of toxin-antitoxin systems in Mycobacterium tuberculosis.
Validation of PRIMA-1MET and TALEN as a therapeutic tools for visual deficient EEC syndrome – PRIMA-TALEN VISION
We aim at evaluating in vitro (3D culture of patient cells) and in vivo (animal model of EEC) the ability of the drug PRIMA-1 to restore visual deficiency of patients affected by EEC ectodermal dysplasia syndrome.<br />
Optimising CNS-targeted neurotensin-peptide-vector conjugates for induction of therapeutic hypothermia – VEC2Brain
consortium for the discovery, optimisation and in vivo validation of peptide-vectors (pVectors) that target [...] ed in BBB endothelial cells, ii) allows RMT in vitro of small organic molecules, peptides and antibodies [...] the development of novel analogues with higher in vitro binding affinity to the LDLR target receptor, increased
Role of the PML nuclear bodies-associated antiviral response in the latent herpes simplex virus type 1 genome patterns acquisition – VIRUCEPTION
The comprehension of the molecular mechanisms that contribute to the establishment then maintenance of a persistent virus in its lethargic state is essential to understand the reasons for the resurgence of the associated disease once the virus reactivates. The VIRUCEPTION project is designed to understand one of key aspects of the interaction between a persistent virus, the herpes simplex virus 1 (HSV-1), and the host cell, i.e. the antiviral role of nuclear bodies.